Gene Construction Kit



Chromium Single Cell Gene Expression provides single cell transcriptome 3' gene expression and multiomic capabilities to profile tens of thousands of cells. Explore cellular heterogeneity, novel targets, and biomarkers with combined gene expression, surface protein expression, or CRISPR edits in each cell.

Construction kits torrentKit

The Ambion Silencer siRNA Construction Kit is for the synthesis of siRNAs by in vitro transcription of DNA templates and costs less per siRNA than chemical synthesis. The kit includes sufficient reagents to synthesize 15 siRNAs. Cut siRNA preparation timegenerate up to 15 siRNAs in less than 24 hou. SnapGene reads.gcc files created by Gene Construction Kit, preserving captions and displaying beautiful, detailed, easy to read maps. Home » Features » Convert File Formats » Gene Construction Kit. SnapGene and SnapGene Viewer can read files created by Gene Construction Kit. Shown below is an example of a GCK file viewed with the original. Integrons and gene cassettes: a genetic construction kit for bacteria Peter M. Bennett Bristol Centre for Antimicrobial Research and Evaluation (BCARE), University of Bristol, Department of Pathology and Microbiology, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK. The construction of overproducing E. Coli strains is a difficult and time-consuming task, primarily because of two factors: (1) the requirement that the gene or complementary DNA to be expressed be refashioned from a molecularly monoclonal source, and (2) the requirement to precisely assemble specific DNA sequences during construction of the.

IDT cloning vectors

Tool Kit Gene

Gene construction kit catalina

All of our synthetic gene products are provided in an optimized cloning vector that is ready to be transformed into E. coli. To optimize manufacturing and delivery times, synthetic genes ≤5 kb in length are delivered in a “best-fit” vector (Table 1), for which you can choose between ampicillin or kanamycin selection markers. Upon receiving your gene, you can then subclone it into the vector of your choice using a variety of methods. The identity of the cloning vector used, its sequence, and insertion site will be confirmed in the documentation that accompanies your product.

Table 1. Best-fit vectors for Genes ≤5000 bp

Gene Construction Kit For Mac

Vector nameVector sizeSelection markerApplicationSequence
pUCIDT (Amp)2752AmpicillinCloningpUCIDT (Amp).txt
pUCIDT (Amp) Golden Gate2752AmpicillinCloningpUCIDT (Amp) Golden Gate.txt
pUCIDT (Kan)2705KanamycinCloningpUCIDT (Kan).txt
pIDTSmart (Amp)2056AmpicillinCloningpIDTSmart (Amp).txt
pIDTSmart (Kan)1962KanamycinCloningpIDTSmart (Kan).txt
pBRIDT3237AmpicillinCloningpBRIDT.txt